The modality of enterobacterial common antigen polysaccharide chain lengths is regulated by o349 of the wec gene cluster of Escherichia coli K-12.

Assembly of cyclic enterobacterial common antigen in Escherichia coli K-12.

We describe here the purification and quantification of a water-soluble cyclic form of enterobacterial common antigen (ECA(CYC)) from Escherichia coli K-12 as well as information regarding its subcellular location and the genetic loci involved in its assembly. Structural characterization of purified ECA(CYC) molecules obtained from E. coli K-12 revealed that they uniformly contained four trisac...

Identification and biosynthesis of cyclic enterobacterial common antigen in Escherichia coli.

Phosphoglyceride-linked enterobacterial common antigen (ECA(PG)) is a cell surface glycolipid that is synthesized by all gram-negative enteric bacteria. The carbohydrate portion of ECA(PG) consists of linear heteropolysaccharide chains comprised of the trisaccharide repeat unit Fuc4NAc-ManNAcA-GlcNAc, where Fuc4NAc is 4-acetamido-4,6-dideoxy-D-galactose, ManNAcA is N-acetyl-D-mannosaminuronic a...

O-ACETYLATION OF THE ENTEROBACTERIAL COMMON ANTIGEN (ECA) POLYSACCHARIDE IS CATALYZED BY THE PRODUCT OF THE yiaH GENE OF Escherichia coli K-12

Molecular typing of avian Escherichia coli isolates by enterobacterial repetitive intergenic consensus sequences-polymerase chain reaction (ERIC-PCR)

BACKGROUND: Colibacillosis is one of the most economically important diseases of poultry worldwide. OBJECTIVES: This study was conducted to examine the clonal relatedness and typing of 95 avian Escherichia coli isolates by ERIC-PCR. METHODS: Sixty-three E. coli isolates from two common manifestations of colibacillosis (yolk sac infection and colisepticemia) and 32 isolates from feces of apparen...

Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) Genotyping of Escherichia coli Strains Isolated from Different Animal Stool Specimens

Background: Escherichia coli is a commensal-pathogenic organism, which includes a wide range of strains. Despite several advanced molecular-genomic technologies for detecting and identifying different strains of E. coli, Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) technique is ...